swing buckets Search Results


sw32 ti swinging bucket rotor  (Danaher Inc)
96
Danaher Inc sw32 ti swinging bucket rotor

Sw32 Ti Swinging Bucket Rotor, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sw32 ti swinging bucket rotor/product/Danaher Inc
Average 96 stars, based on 1 article reviews
sw32 ti swinging bucket rotor - by Bioz Stars, 2026-04
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sw41ti swing out rotor  (Beckman Coulter)
96
Beckman Coulter sw41ti swing out rotor
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Sw41ti Swing Out Rotor, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sw41ti swing out rotor/product/Beckman Coulter
Average 96 stars, based on 1 article reviews
sw41ti swing out rotor - by Bioz Stars, 2026-04
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centrifuge  (Eppendorf AG)
92
Eppendorf AG centrifuge
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/centrifuge/product/Eppendorf AG
Average 92 stars, based on 1 article reviews
centrifuge - by Bioz Stars, 2026-04
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swinging bucket rotor  (Danaher Inc)
93
Danaher Inc swinging bucket rotor
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Swinging Bucket Rotor, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/swinging bucket rotor/product/Danaher Inc
Average 93 stars, based on 1 article reviews
swinging bucket rotor - by Bioz Stars, 2026-04
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js 5 3 allspin swinging bucket rotor  (Danaher Inc)
93
Danaher Inc js 5 3 allspin swinging bucket rotor
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Js 5 3 Allspin Swinging Bucket Rotor, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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js 5 3 allspin swinging bucket rotor - by Bioz Stars, 2026-04
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open top thinwall tube beckman coulter  (Danaher Inc)
96
Danaher Inc open top thinwall tube beckman coulter
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Open Top Thinwall Tube Beckman Coulter, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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ultra centrifuge tubes  (Danaher Inc)
93
Danaher Inc ultra centrifuge tubes
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Ultra Centrifuge Tubes, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assembly  (Danaher Inc)
93
Danaher Inc assembly
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Assembly, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
assembly - by Bioz Stars, 2026-04
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swinging bucket  (Danaher Inc)
93
Danaher Inc swinging bucket
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Swinging Bucket, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/swinging bucket/product/Danaher Inc
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centrifuge allegra x 14r  (Beckman Coulter)
93
Beckman Coulter centrifuge allegra x 14r
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Centrifuge Allegra X 14r, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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js 7 5 rotor  (Beckman Coulter)
93
Beckman Coulter js 7 5 rotor
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Js 7 5 Rotor, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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swing out buckets  (Beckman Coulter)
95
Beckman Coulter swing out buckets
Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
Swing Out Buckets, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: STAR Protocols

Article Title: Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

doi: 10.1016/j.xpro.2023.102248

Figure Lengend Snippet:

Article Snippet: SW32 Ti Swinging-Bucket Rotor , Beckman Coulter , 369694.

Techniques: Recombinant, Saline, dsDNA Assay, Software

Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a SW41Ti rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.

Journal: Journal of Extracellular Vesicles

Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures

doi: 10.3402/jev.v3.23592

Figure Lengend Snippet: Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a SW41Ti rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.

Article Snippet: The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).

Techniques: Gradient Centrifugation, Expressing, Centrifugation, Derivative Assay, Negative Control, Software, Purification, Incubation, Magnetic Beads